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Mass spectrometric analysis of sulfur mustard-induced biomolecular adducts: Are DNA adducts suitable biomarkers of exposure?

TitleMass spectrometric analysis of sulfur mustard-induced biomolecular adducts: Are DNA adducts suitable biomarkers of exposure?
Publication TypeJournal Article
Year of Publication2018
AuthorsZubel T., Burkle A., Mangerich A.
JournalToxicol Lett
Volume293
Pagination21-30
Date PublishedSep 1
ISBN Number0378-4274 (Linking)
Accession Number29277573
KeywordsAnimals, Biomarkers, Biomarkers/*analysis, Chemical Warfare, CHEMICAL WARFARE AGENTS, Chemical Warfare Agents/*toxicity, Chromatography, High Pressure Liquid, DNA Adducts/*analysis, DNA Damage, Humans, LC-MS/MS, Mass Spectrometry, Mustard Gas/*toxicity, Sulfur Mustard, Tandem Mass Spectrometry, Technical review
Abstract

The bi-functional chemical warfare agent sulfur mustard (SM), whose release in asymmetric conflicts or terrorist attacks represents a realistic threat, induces several kinds of biomolecular adducts, including highly toxic DNA adducts. Isotope dilution liquid chromatographic tandem mass spectrometry (ID-LC-MS/MS) is considered the gold standard for highly accurate, precise, specific and sensitive quantification of DNA adducts in general. Recently, a number of LC-MS/MS approaches have been established to analyze SM-induced protein and DNA adducts in cell culture and rodent animal models. As DNA adducts are mechanism-based biomarkers for SM exposure, results from such studies provide a deeper understanding of the etiology of SM-induced pathologies, especially of long-term effects such as cancer formation. As a result, medical treatment of SM-exposed individuals might be improved. Yet, despite the progress that has been made during the last years, there is still a need for advanced methods of ID-LC-MS/MS for the detection and quantitation of SM adducts.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/29277573
DOI10.1016/j.toxlet.2017.12.014
Short TitleMass spectrometric analysis of sulfur mustard-induced biomolecular adducts: Are DNA adducts suitable biomarkers of exposure?

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