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Evaluation of mRNA Expression Levels of TNFalpha, TNFR1 and IL1beta in Lung Tissue 20 Years after Sulfur-mustard Exposure

TitleEvaluation of mRNA Expression Levels of TNFalpha, TNFR1 and IL1beta in Lung Tissue 20 Years after Sulfur-mustard Exposure
Publication TypeJournal Article
Year of Publication2018
AuthorsEghtedardoost M., Hassan Z.M, Ghazanfari T., Sadeghipour A., Ghanei M.
JournalIran J Allergy Asthma Immunol
Volume17
Issue4
Pagination379-387
Date PublishedAug 12
ISBN Number1735-1502 (Linking)
Accession Number30537801
KeywordsInterleukin-1 beta, LUNG, mRNA expression, Sulfur Mustard, Tumor necrosis factor receptor type 1, Tumor Necrosis Factor-alpha
Abstract

Despite many years having passed since exposure to sulfur mustard (SM) gas, there are many exposed subjects who are still suffering from delayed pulmonary complications. The levels of pro-inflammatory cytokines in the lung of these subjects have not been investigated in delay phase. In this study, we evaluated mRNA expression of pro-inflammatory cytokines (tumor necrosis factor alpha (TNFalpha), tumor necrosis factor receptor type 1 (TNFR1), and interleukin 1 beta (IL-1beta) ) in lung biopsy of SM-exposed subjects and compared them with control (non-exposed) subjects. We used formalin-fixed, paraffin-embedded (FFPE) tissue for this purpose. Lung FFPE blocks of SM-exposed subjects (30 samples) and a control group (30 samples) were collected from archival pathology department. The total mRNA of FFPE tissues were extracted and the mRNA expression of pro-inflammatory cytokines were determined by quantitative Real Time PCR (RT-qPCR). The obtained results from two groups were compared to each other and non-parametric statistical analyses were carried out on them. Our studies showed that the mRNA expression of TNFalpha, TNFR1 and IL-1beta in lung tissue of SM injured and control people have no significant difference (p-value= 0.159, 0.832 and 0.314 respectivly). TNFR1 showed direct correlation with TNFalpha (r=0.867, p=0.002) and IL-1beta (r= 0.65, p=0.006). The evaluation of mRNA expression in pro-inflammatory cytokines in lung of SM-exposed subjects after 20 years showed that these mediators are similar to those of non-exposed group and there was no acute inflammation in lung of these patients.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/30537801
DOI10.18502/ijaai.v17i4.97
Short TitleEvaluation of mRNA Expression Levels of TNFalpha, TNFR1 and IL1beta in Lung Tissue 20 Years after Sulfur-mustard Exposure

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