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Sulfur mustard causes oxidants/antioxidants imbalance through the overexpression of free radical producing-related genes in human mustard lungs

TitleSulfur mustard causes oxidants/antioxidants imbalance through the overexpression of free radical producing-related genes in human mustard lungs
Publication TypeJournal Article
Year of Publication2016
AuthorsE. Marzony T, Nejad-Moghadam A., Ghanei M., Panahi Y.
JournalEnviron Toxicol Pharmacol
Volume45
Pagination187-92
Date PublishedJul
ISBN Number1382-6689 (Linking)
Accession Number27317991
Keywords*Oxidative Stress/drug effects/genetics, Aldehyde Oxidase/genetics, Antioxidants/*metabolism, Armed Conflicts, Bronchoalveolar Lavage Fluid/chemistry, Case-Control Studies, Chemical Warfare Agents/*toxicity, Dual Oxidases, Eosinophil Peroxidase/genetics, Gene Regulatory Networks/*drug effects, Humans, Iodide Peroxidase/genetics, Lung tissue, Lung/*drug effects/metabolism/pathology, Male, Middle Aged, Mustard Gas/*toxicity, NADPH Oxidases/genetics, Oxidants/antioxidants imbalance, Oxidative stress, Peroxidase/genetics, Reactive Oxygen Species/metabolism, Respiratory Function Tests, ROS producing-related genes, Sulfur Mustard
Abstract

The aim of this study is to analyze oxidative stress (OS) and changes in expression of reactive oxygen species (ROS) producing-related genes in mustard lungs. Human lung biopsies provided from controls (n=5) and sulfur mustard (SM)-exposed patients (n=6). Changes in expression of dual oxidases (DUOXs), aldehyde oxidase 1 (AOX1), thyroid peroxidase (TPO), myeloperoxidase (MPO) and eosinophil Peroxidase (EPO) were measured using RT(2) Profiler() PCR Array. OS was evaluated by determining bronchoalveolar lavage fluids (BALF) levels of total antioxidant capacity (TAC) and malondialdehyde (MDA). Higher TAC value was observed in BALF of controls compared with patients (0.138+/-0.02683mumol/l vs 0.0942+/-0.01793mumol/l), whereas a significant increase in MDA concentration was found in patients (0.486+/-0.04615 nmol/l vs 0.6467+/-0.05922 nmol/l). All ROS producing-related genes were overexpressed in the order AOX1>MPO>DUOX2>DUOX1>TPO>EPO. Upregulation of these genes may be a reason for overproduction of ROS, oxidants/antioxidants imbalance, OS and respiratory failures in mustard lungs.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/27317991
DOI10.1016/j.etap.2016.06.001
Short TitleSulfur mustard causes oxidants/antioxidants imbalance through the overexpression of free radical producing-related genes in human mustard lungs

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