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The advantage of using the hairless guinea pig (HGP) as a small animal model for studying healing processes following HD skin injury

TitleThe advantage of using the hairless guinea pig (HGP) as a small animal model for studying healing processes following HD skin injury
Publication TypeConference Proceedings
Year of Conference2006
AuthorsDachir S., Cohen M., Fishbeine E., Gutman H., Kadar T.
Conference NameProceedings of the U.S. Army Medical Defense Bioscience Review
Pagination89
Conference LocationHunt Valley, MD
Abstract

Skin exposure to sulfur mustard results in erythema, edema and severe injury, which take a long time to heal. Despite many years of research, the only recommended treatment for HD induced skin injury, is the use of anti-inflammatories that were proved to significantly reduce erythema and edema. However, the cytotoxic effects of HD causing damage to the skin were only slightly ameliorated. Several in vivo models for skin exposure to HD were developed over the years one of which was the hairless guinea pig (HGP). The aim of this study was to further characterize the effects of HD vapor on HGP skin injury and healing processes, and to test the efficacy of anti-inflammatory treatments. HGP's were exposed to HD vapor for 1-30 min. Peak erythema values were measured about 6 hr. post-exposure and TEWL started to increase at 72 hr. Prostaglandin E content and metalloproteinase-9 activity increased as soon as 2 hr. post-exposure. Histological examination exhibited impaired epithelial regeneration and impaired basement membrane. Post-exposure treatment with anti-inflammatories reduced erythema significantly and prevented the increase in PGE. These results support the use of the HGP as an in-vivo model for long-term effects of HD on skin injury as well as for studying treatment effects.

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