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Molecular similarities of invasive epithelial carcinoma cells and sufur mustard-induced wounds

TitleMolecular similarities of invasive epithelial carcinoma cells and sufur mustard-induced wounds
Publication TypeConference Proceedings
Year of Conference2010
AuthorsChang Y-C, Soriano M., Wang J., Gordon M.K., Sabourin C.L, Casillas R.P, Gerecke D.R
Conference NameProceedings of the 2010 U.S. Army Medical Defense Bioscience Review
Conference LocationHunt Valley, MD

There are several distinct phases during normal incisional or thermal wound repair including coagulation, inflammation, migration/proliferation, and remodeling. These stages are distinguished by specific inflammatory cell recruitment that occurs in a somewhat predictable pattern. However, in sulfure mustard exposed skin, these stages are altered, inflammation is prolonged, and there is remodeling of the usual de novo skin structures repaired within the first week post exposure. In addition the temporal. and spatial expression of normal wound repair molecules may be altered due to the prolonged inflammation. Immunohistochemical and RT-PCR studies were performed and markers of skin injury and repair examined for various time-points after sulfur mustard exposure using the mouse ear vesicant model. We observed the co-expression of laminin y2 and the cell senescence protein, p16 in the epithelial cells at the migrating front of the epithelial sheet at the wound bed. In contrast the hyperproliferating cells behind the migrating front were abundant in both keratin 5, an intermediate filament protein of proliferating keratinocytes and Ki67, a cell cycle marker of the growth stage. These are the same molecules expressed by invasive epithelial carcinoma cells. These observations may provide information regarding novel molecular targets for medical countermeasures against sulfur mustard damage or alternatively, identify targets that might enhance the wound repair process while minimizing the potential for scarring. This work is supported by NIH grants ES005022 and AR055073.

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